Female oocyte Fertilized oocyte (zygote), there are 2 male and female pronuclei in the center of oocyte  4-cell embryo

Essence of the in vitro fertilization (IVF) procedure is to get mature oocytes from woman ovaries and to fertilize these oocytes with spermatozoa of husband (or donor's sperm if both spouses agree) and to rise created embryos in the incubator during 48-72 hours period with following embryo transfer to the patient's uterus. Starting from 2nd day of the menstrual cycle patient receives stimulating tablets and injections. It helps to put into ovaries and to rise as a rule not one but few oocytes. In ultrasonic research of ovaries and an uterus conducted approximately from the day 8th to the day 14th, a doctor looks after the growth of the follicles in ovaries and oocytes maturing in it. At some point of the cycle (from 10th to 16th) injection is prescribed (usually at night 23.00), that determines the moment of the final maturing and punction of follicles. In 36 hours after that injection all oocytes are taken from the patient (Fig.1) (these oocytes are sucked with thinnest needle) and at the same day man gives his sperm for IVF completion. Before fertilization the sperm being handled carefully to get as much active spermatozoa as possible, to clean them of semen plasma, leukocytes and other sperm elements which can affect the fertilization. Received after the processing, spermatozoa will be added to the wife's oocytes. Oocytes staying in the nutrient medium in special 4-well multi dishes. Then, these dishes with oocytes and spermatozoas (with detailed data about husband and wife) will be placed to the incubator. Incubator electronic devices control strictly the temperature, humidity, gas environment level, which is necessary for normal fertilization and embryo growth. In 18 hours embryologist picks dishes from the incubator to define fertilization availability. If fertilization completed successfully (fig.2) oocytes will be putted into special nutrient medium where oocytes cleavage take place, means become embryos. This process takes 16-18 hours too. Embryo transfer to the patient's uterus will be done (Fig.3), if the embryologist sees normally growing embryos. Embryos should become acclimatize in the uterus. In a case of success in 3 weeks after embryo transfer ultrasonic research take place to check the number of settled down embryos.